|
KMID : 0369819950250040299
|
|
Jorunal of Korean Pharmaceutical Sciences
1995 Volume.25 No. 4 p.299 ~ p.312
|
|
|
Functional Expression of a Dipeptide Transporter Obtained from Intestinal HT-29 Cells Using Xenopus Oocytes
|
|
|
|
|
Abstract
|
|
|
Cloning the gene encoding a dipeptide transporter is necessary for understanding the absorption mechanism of peptides and peptide-like drugs in the gastrointestinal tract. Functional expression of a dipeptide transporter after microinjection into
Xenopus laevis oocytes was performed using the mRNA purified from human intestinal HT-29 cells. Fifty nanoliters of purified mRNA(1mg/mL) were microinjected into healthy oocytes followed by incubation for 4 days in order to express a dipeptide
transporter. Functional expression was determined by a uptake assay using 10Ci/mL [3H]-glycylsarcosine, a dipeptide substate of the transporter. Seasonal variability and batch-to-batch variability were greater in summer. The usage of beveled
micropipettes improves viability of oocytes at 4 days after microinjection. Expression of a dipeptide transporter in oocytes after microinjection of mRNA obtained from HT-29 cells was significantly larger than those after microinjection of water
on
mRNA
obtained from the rabbit intestine.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|